The ophiolites from the Yarlung Zangbo River (Tibet),Southwestern China,were analysed for the con-tents of helium and neon and their isotopic compositions by stepwise heating. The serpentinites from Bainang showed a high 3He/4He value of 32.66Ra (Ra is referred to the 3He/4He ratio in the present air) in 700 ℃ fraction. At lower temperature,all of the dolerites displayed as very high 3He/4He ratios as ones investigated for hotspots. It was clear that the high 3He/4He ratio was one of immanent characterics in the magma source formed the dolerites,suggesting that there was a large amount of deep mantle fluids in these rocks. In the three-isotope diagram of neon,the data points from the ophiolites of the Yarlung Zangbo River were arranged along the Loihi Line. This is in agreement with the characteristics of he-lium isotopes,revealing that the high-3He plume from deep mantle had played an important role in the formation of the Neo-Tethyan Ocean. The helium isotopic compositions in the basalts were far higher than atomospheric value but lower than the average value of MORB,although there were various de-grees of alteration. The possible reasons were that basaltic magmas The ophiolites from the Yarlung Zangbo River (Tibet), Southwestern China, were analyzed for the con-tents of helium and neon and their isotopic compositions by stepwise heating. The serpentinites from Bainang showed a high 3He / 4He value of 32.66 Ra (Ra is referred to the 3He / 4He ratio in the present air) at 700 ° C fraction. At lower temperature, all of the dolerites displayed as very high 3He / 4He ratios as ones checked for hotspots. It was clear that the high 3He / 4He ratio was one of immanent characterics in the magma source formed the dolerites, suggesting that there was a large amount of deep mantle fluids in these rocks. In the three-isotope diagram of neon, the data points from the ophiolites of the Yarlung Zangbo River were arranged along the Loihi Line. This is in agreement with the characteristics of he-lium isotopes, revealing that the high-3He plume from deep mantle had played an important role in the formation of the Neo-Tethyan Ocean. The helium isotopic compositions in the b asalts were far higher than atomospheric value but lower than the average value of MORB, although there were various de-grees of alteration. The possible reasons were that basaltic magmas