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目的 :明确冬眠心肌细胞的超微结构改变。方法 :利用 2 2只犬 ,使冠状动脉左前降支内径缩窄约 4 8% ,造成节段性室壁运动异常 ,继续喂养 ( 4 5 2± 9 6)d ,建立慢性心肌缺血而无坏死的动物模型 ,并进行光镜及电镜检查 ,观察慢性冬眠心肌细胞结构的改变。结果 :10只犬完成了所有实验步骤 ,成功地建立了慢性心肌冬眠的动物模型 ,10只犬均出现了不同程度的心肌细胞形态学改变。结论 :慢性冬眠心肌细胞存在着心肌纤维、线粒体、细胞间质和细胞核不同程度的结构损害。
Objective: To clarify the ultrastructural changes of hibernating cardiomyocytes. Methods: Twenty-two canines were used to narrow the diameter of the left anterior descending coronary artery by 48%, resulting in segmental wall motion abnormalities and continuing feeding (452 ± 96 days) to establish chronic myocardial ischemia without Necrosis of animal models, and light and electron microscopy to observe changes in the structure of chronic hibernating myocardial cells. Results: All the 10 dogs completed all the experimental steps and successfully established animal models of chronic myocardial hibernation. All of the 10 dogs showed morphological changes of myocardial cells to varying degrees. Conclusion: There are some structural damage of myocardial fibrosis, mitochondria, interstitial cells and nucleus in chronic hibernating myocardium.