【摘 要】
：
通过电化学法研究了fsDNA影响下芹菜素(API)在金电极上的电极行为,优化确定了fsDNA的检测条件。结果表明,在4.0×10-7~7.0×10-6g/mL浓度范围内峰电流的增强与加入的fsDNA的
【机 构】
：
中国矿业大学化工学院,潍坊学院化学化工与环境工程学院,
论文部分内容阅读
通过电化学法研究了fsDNA影响下芹菜素(API)在金电极上的电极行为,优化确定了fsDNA的检测条件。结果表明,在4.0×10-7~7.0×10-6g/mL浓度范围内峰电流的增强与加入的fsDNA的浓度呈线性关系,线性方程为ΔIp/(μA)=3.20CfsDNA(10-5g/mL)+1.06,检测限为5.8×10-8g/mL,对模拟样品的测定结果令人满意;研究还表明芹菜素发生了3e传递行为,并有质子参与,且该行为受到扩散控制。通过循环伏安、紫外-可见光谱等方法对API与fsDNA间的作用机制进行了初步探讨,结果表明两者之间存在着静电作用,结合常数K=1.8×104L/mol。
The electrode behavior of apigenin (api) on gold electrode was studied by electrochemical method and the detection conditions of fsDNA were optimized. The results showed that there was a linear relationship between the increase of peak current and the concentration of fsDNA in the concentration range of 4.0 × 10-7 ~ 7.0 × 10-6g / mL, the linear equation was ΔIp / (μA) = 3.20CfsDNA (10-5g / mL) +1.06 with a detection limit of 5.8 × 10-8g / mL. The results of the determination of simulated samples are satisfactory. The study also shows that 3e transfer of apigenin takes place and proton is involved, and this behavior is controlled by diffusion. The mechanism of interaction between API and fsDNA was studied by cyclic voltammetry and ultraviolet-visible spectroscopy. The results showed that there was an electrostatic interaction between the two and the binding constant was K = 1.8 × 104L / mol.
其他文献
采用共沉淀法制备了CeO2,Co3O4和一系列Co3O4/CeO2复合氧化物催化剂,在400°C下含SO2的氧化气氛中对催化剂进行了硫中毒处理,通过原位红外光谱、X射线衍射、程序升温脱附和X
为探讨风湿性心脏病（RHD）患者风湿热复发（RARF）的诊断，选择具2年以上病程的RHD患者和2次超声心动图检查且本次检查显示心脏瓣膜病变比2年前有明显加重的97例病人，认为RARF的诊断条件是：在新近A组链球菌感染基础上，RHD患者具有Jones标准一项主要表现，或缺乏主要表现而具备次要表现五项中之两项以上者即可诊断为RARF。若仅具备次要表现一项，并伴有贫血、腹痛、鼻衄、心前区隐痛等症状两项和
Mesoporous CeO2-MnOx binary oxides with different Mn/Ce molar ratios were prepared by hydrothermal synthesis and characterized by scanning electron microscopy(S
合成了(丙烯酸)二(α-噻吩甲酰三氟丙酮)钐[Sm(TTA)_2AA)]配合物,并通过本体聚合合成了Poly(MMA-co-Sm(TTA)_2AA)光致发光材料,采用红外光谱分析、示差扫描量热法、荧光光谱
本文结合我国目前水资源所存在的问题和功能区划的重要性,对水功能区划的原则、方法和分类进行阐述。并对水功能区的管理给出几点建议。
Based on the current problems of
A facile approach for the first total synthesis of naturally occurring geranylated flavanoids sepicanin A has been obtained with total yield 16%starting from 2,
A nanoheterojunction composite photocatalyst Bi2O3/TiO2working under visible-light(λ 420 nm) was prepared by combining two semiconductors Bi2O3 and TiO2 varyin
考虑到盐穴储气库群间矿柱失稳破坏可以看成矿柱变形从连续逐渐变化到系统发生突变的过程,是一种典型的非线性突变问题.本文建立了盐穴储气库群间矿柱稳定性分析的尖点位移突
The novel hyperbranced polymers containing reductive groups were successfully prepared and characterized using redox/reversible addition fragmentation chain tra
1纺织用聚酯的内部回收——驱动力目前,主要的现代化纺织用聚酯生产厂中,高产能生产线直接纺长丝或短纤维的日产量大约在400~800 t。尤其在中国,在单个生产地点,公司拥有多条