将大约0.8g 已经冲洗和干燥过的羟基磷灰石(hydroxyapatite,HA)装入1ml 塑料注射器至0.8ml 处,制成无菌柱,用5ml 含有5%热灭活胎牛血清的Dulbecco PBS 冲洗备用。健康人肝素抗凝静脉血经过Ficoll-Hypaque 密度梯度离心获取PBL。取0.2ml(0.5～1×10~7个)装柱,以5ml37℃RPMI—1640培基缓慢冲洗,将约5ml 洗脱液收集 Approximately 0.8 g of hydroxyapatite (HA), which had been rinsed and dried, was loaded into a 1 ml plastic syringe to 0.8 ml to make a sterile column and rinsed with 5 ml of Dulbecco’s PBS containing 5% heat-inactivated fetal bovine serum spare. Healthy human heparin anticoagulated venous blood was obtained by Ficoll-Hypaque density gradient centrifugation to obtain PBL. Take 0.2ml (0.5 ~ 1 × 10 ~ 7) packed column, wash slowly with 5ml RPMI-1640 culture medium at 37 ℃ and collect about 5ml eluate