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目的探讨流式细胞术检测血小板相关抗体在特发性血小板减少性紫癜诊治中的价值。方法用流式细胞术、ELISA法测定45例ITP患者及20例体检正常人的血小板相关抗体(PAIgG、PAIgM、PAIgA)的水平,并分别进行ITP患者与正常人进行配对比较。结果 FCM检测20例正常人血小板表面IgG、IgA和IgM的荧光百分率分别为(15.21±8.83)%,(4.89±7.48)%,(13.51±6.21)%;ITP患者血小板表面IgG、IgA和IgM荧光百分率分别为(53.21±18.92)%,(45.95±20.21)%,(22.18±15.77)%。结论血小板特异抗体检测对肯定ITP诊断有重要的意义,流式细胞术可快速、敏感、准确地测定PAIg,且明显优于ELISA法。
Objective To investigate the value of flow cytometry in the diagnosis and treatment of idiopathic thrombocytopenic purpura. Methods The levels of platelet-associated antibodies (PAIgG, PAIgM, PAIgA) in 45 patients with ITP and 20 healthy controls were measured by flow cytometry and ELISA, and the ITP patients were compared with normal subjects respectively. Results The percentages of IgG, IgA and IgM on the surface of platelets detected by FCM were (15.21 ± 8.83)%, (4.89 ± 7.48)%, (13.51 ± 6.21)%, respectively. The platelet surface IgG, IgA and IgM fluorescence The percentages were (53.21 ± 18.92)%, (45.95 ± 20.21)% and (22.18 ± 15.77)%, respectively. Conclusion The detection of platelet-specific antibody is of great significance for the diagnosis of ITP. Flow cytometry can detect PAIg rapidly, sensitively and accurately, and is superior to ELISA.