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目的:探讨补充赖氨酸对一次力竭运动大鼠肝脏SOD、MDA和细胞凋亡的影响。方法:将24只SD大鼠随机分成3组:安静对照组、运动组和运动+给药组。运动大鼠均采用Bedford所建立的运动负荷模型进行一次力竭运动;给药组大鼠力竭运动前灌胃L-赖氨酸0.1ml/10g体重。力竭运动后即刻取材,用TUNEL法检测肝细胞凋亡,比色法检测肝组织SOD活性和MDA含量。结果:运动组和运动+给药组大鼠力竭时间分别为210.36±37.67min和259.19±38.61min,具有显著性差异(P<0.05);运动组和运动+给药组肝细胞凋亡指数均显著高于安静对照组(P<0.01,P<0.05),但运动+给药组显著低于运动组(P<0.05);运动组和运动+给药组大鼠肝脏MDA含量显著高于安静对照组(P<0.05),运动+给药组显著低于运动组(P<0.05);运动组和运动+给药组大鼠肝脏SOD活力显著低于安静对照组(P<0.05),运动+给药组显著高于运动组(P<0.05)。结论:一次力竭运动可导致大鼠肝组织损伤,诱导肝细胞凋亡;L-赖氨酸对急性力竭运动大鼠肝组织有保护作用。
Objective: To investigate the effects of lysine supplementation on liver SOD, MDA and apoptosis in rats after exhaustive exercise. Methods: Twenty-four SD rats were randomly divided into 3 groups: control group, exercise group and exercise + administration group. Rats in exercise group were subjected to exhaustive exercise using Bedford’s exercise load model. Rats in treatment group were given L-lysine 0.1 ml / 10 g body weight before exhaustive exercise. Immediately after exhaustive exercise, the hepatocytes were harvested by TUNEL assay. The activity of SOD and the content of MDA in liver tissue were detected by colorimetry. Results: The exhaustion time of exercise group and exercise + administration group was 210.36 ± 37.67min and 259.19 ± 38.61min, respectively (P <0.05), and the indexes of apoptosis in exercise group and exercise + administration group (P <0.01, P <0.05), but the exercise + administration group was significantly lower than that in the exercise group (P <0.05). The MDA content in the exercise group and exercise + administration group was significantly higher than that of the control group (P <0.05), and the activity + administration group was significantly lower than that of the exercise group (P <0.05). The SOD activity of the liver of the exercise group and exercise + administration group was significantly lower than that of the control group (P <0.05) Exercise + administration group was significantly higher than exercise group (P <0.05). Conclusion: A single exhaustive exercise can induce liver injury in rats and induce apoptosis of liver cells. L-Lysine can protect liver from acute exhaustive exercise.