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目的寻找木糖氧化无色杆菌噬菌体简便高效的保存方法。方法将该噬菌体用不同的方法和温度保存,在不同时间点检测噬菌体的效价。结果与结论在-80℃、-20℃和4℃温度保存时,甘油和二甲基亚飒保存16个月后,phi Axp-1和phi Axp-2效价能稳定在初始的1010PFU/ml,phi Axp-3效价略微从1011下降到1010PFU/ml。使用氯仿在4℃保存16个月后,3株噬菌体的效价都略微下降,但优于其他保存温度(-80℃、-20℃、室温和37℃)。因此,这些都是保存木糖氧化无色杆菌噬菌体的合适方法。
Objective To find a simple and efficient method for the preservation of xylose-free Achromobacter xylophilus phage. Methods The phages were stored in different ways and at different temperatures, and phage titer was measured at different time points. Results and Conclusion The potency of phi Axp-1 and phi Axp-2 after 16 and 12 months of glycerol and dimethyl sulfoxide storage at -80 ° C, -20 ° C, and 4 ° C, stabilized at an initial 1010 PFU / ml , the phi Axp-3 titer decreased slightly from 1011 to 1010 PFU / ml. After 16 months of storage at 4 ° C with chloroform, the titer of the 3 phages decreased slightly, but was superior to the other storage temperatures (-80 ° C, -20 ° C, room temperature and 37 ° C). Therefore, these are all suitable methods for preserving Achromobacter xylose bacteriophages.