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多源耐药基因的过度表达是导致白血病化学药物治疗失败的主要原因之一。在正常细胞组织中多源耐药基因的功能是使编码细胞膜蛋白,防止脂类物质在细胞中的堆积及甾体激素的转运。由于细胞膜蛋白及其mRNA在细胞中含量极低,应用传统的蛋白质检测手段及Northern blot检测其水平不够敏感。本研究建立了应用逆转录PCR技术对多源耐药基因的定量检测,其方法灵敏、快速,具有极大的临床应用价值。
The overexpression of multi-drug resistance genes is one of the main reasons for the failure of chemotherapy of leukemia. The function of multi-drug resistant genes in normal cellular tissues is to encode cell membrane proteins, prevent lipid accumulation in cells, and steroid hormone transport. Due to the extremely low content of cell membrane protein and its mRNA in cells, it is not sensitive enough to use traditional protein detection methods and Northern blot to detect its level. This study established a quantitative reverse transcription PCR detection of multi-drug resistance gene, the method is sensitive and rapid, with great clinical value.