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目的建立离子交换-电导检测离子色谱法,同时测定饮料中的甜蜜素、山梨酸、苯甲酸、安赛蜜和糖精钠的含量。方法选用IonPacRAS194×250mm为分析柱,IonPacRAS194×50mm为保护柱,18mM氢氧化钾作为淋洗液,流速1.0ml/min,样品经适当稀释滤膜过滤后进行色谱分析。结果方法的线性范围广、相关性好(R2均﹥0.999),甜蜜素、山梨酸、苯甲酸、安赛蜜和糖精钠测定的相对标准偏差(RSD)分别为0.29%～2.96%、0.35%～2.21%、0.40%～2.12%、0.61%～2.09%、0.47%～2.78%,加标回收率分别为97.2%～101.7%、97.0%～103.6%、96.8%～101.2%、97.3%～100.1%,98.1%～102.0%,方法检出限分别为0.40μg/ml、0.50μg/ml、0.50μg/ml、0.10μg/ml、2.0μg/ml。结论该方法简单、快速、准确,且灵敏度高。
Objective To establish an ion exchange - conductivity detection ion chromatography, simultaneous determination of sodium cyclamate, sorbic acid, benzoic acid, acesulfame and saccharin sodium in beverages. Methods IonPacRAS194 × 250mm as analytical column, IonPacRAS194 × 50mm as guard column, 18mM potassium hydroxide as eluent flow rate of 1.0ml / min, the sample was filtered through a suitable membrane chromatography and chromatographic analysis. Results The linear range of the method was good and the correlation was good (R2> 0.999). The relative standard deviations (RSDs) of sodium cyclamate, sorbic acid, benzoic acid, acesulfame and saccharin sodium were 0.29% -2.96% ~ 2.21%, 0.40% ~ 2.12%, 0.61% ~ 2.09% and 0.47% ~ 2.78%, respectively. The recoveries were 97.2% ~ 101.7%, 97.0% ~ 103.6%, 96.8% ~ 101.2%, 97.3% ~ 100.1 %, 98.1% ~ 102.0%. The detection limits were 0.40μg / ml, 0.50μg / ml, 0.50μg / ml, 0.10μg / ml and 2.0μg / ml respectively. Conclusion The method is simple, rapid, accurate and sensitive.