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采用~3H-TdR掺入法和羟基磷灰石层析法,比较了~(60)Coγ射线照射后PHA、ConA、PWM激活的人血淋巴细胞的转化、DNA链断裂及其修复。结果表明,受照后这3种细胞转化受抑,在0～8Gy范围内,剂量效应呈双相线性关系,其中PWM细胞对射线敏感程度最低。3种细胞受照后DNA发生链断裂,在0～30Gy范围内,与剂量呈线性关系,三者之间无显著差异。受15Gy照射后,3种细胞在37℃条件下能修复DNA断链,10min内修复很快,30min修复到高峰,但修复不完全,修复后DNA分子发生再断裂。PWM细胞DNA修复率最高。淋巴细胞转化对辐射的敏感性可能与DNA断链的修复能力有关。
The ~3H-TdR incorporation method and hydroxyapatite chromatography were used to compare the conversion of PHA, ConA, and PWM-activated human blood lymphocytes, DNA strand breaks and their repair after ~(60)Co γ-ray irradiation. The results showed that after irradiation, these three kinds of cells were inhibited. In the range of 0-8 Gy, the dose effect showed a biphasic linear relationship, in which the PWM cells had the lowest sensitivity to radiation. After 3 cells were irradiated, DNA fragmentation occurred in the range of 0-30 Gy, and the dose was linear. There was no significant difference between the three groups. After irradiation with 15Gy, the three cells could repair DNA fragmentation at 37°C, and repaired within 10 minutes. After repairing for 30 minutes, the three cells repaired to a peak, but the repair was incomplete and the repaired DNA molecules re-fragmented. The DNA repair rate of PWM cells is the highest. The sensitivity of lymphocyte transformation to radiation may be related to the ability to repair DNA strand breaks.