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为了观察外周注射福尔马林对大鼠前扣带皮层(ACC)中星形胶质细胞标记物胶质纤维酸性蛋白(GFAP)和S100B,小胶质细胞标记物CD14以及与胶质细胞密切相关的炎症前细胞因子白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)表达的影响,本研究结合行为学检测和RT-PCR方法,观察了大鼠单侧足底皮下注射福尔马林后的行为学变化,并检测了在不同时间点ACC中GFAP、S100B、CD14、IL-1β和TNF-α在基因水平的表达变化。结果显示:福尔马林急性疼痛刺激的大鼠出现典型的双时相自发性疼痛反应。ACC中GFAP和S100B mRNAs表达水平在刺激后30min、1h增高,2h恢复正常;CD14 mRNA表达水平在15min开始增高,1h达到高峰,6h恢复正常;IL-1β和TNF-αmRNAs表达水平在刺激后30min、1h、2h增高,6h恢复正常。上述结果表明,福尔马林外周急性伤害性刺激能够诱导ACC内短暂性的星形胶质细胞、小胶质细胞激活及IL-1β、TNF-α表达增高,提示激活的胶质细胞及表达上调的炎症前细胞因子可能参与伤害性信息的调制。
In order to observe the effect of formalin injection on glial fibrillary acidic protein (GFAP) and S100B (astrocyte markers) in rat anterior cingulate cortex (ACC), microglial marker CD14 and in close proximity with glial cells Related pre-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) expression in this study combined with behavioral testing and RT-PCR method to observe the rat unilateral subcutaneous injection of blessing The changes of behavioral changes after the administration of cortisol and the changes of gene expression of GFAP, S100B, CD14, IL-1β and TNF-α at different time points were detected. The results showed that typical dual-phase spontaneous pain reaction occurred in rats with formalin-induced acute pain. The expression of GFAP and S100B mRNAs in ACC increased at 30 min and 1 h after stimulation, and returned to normal at 2 h. The expression of CD14 mRNA increased at 15 min, peaked at 1 h and returned to normal at 6 h. The expression levels of IL-1β and TNF- , 1h, 2h increased, 6h returned to normal. The above results show that formalin peripheral acute nociceptive stimulation can induce transient astrocyte and microglial activation in ACC as well as increased expression of IL-1β and TNF-α, suggesting activated glial cells and their expression Up-regulated pro-inflammatory cytokines may be involved in the modulation of nociceptive information.