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目的探讨 IL-8及 TNF-α在慢性阻塞性肺疾病 ( COPD)发病机理中的作用。方法收集 3 0例 COPD患者急性发作期及稳定期的痰液。应用 ELISA方法测定痰液中白介素 -8( IL-8)及肿瘤坏死因子 -α( TNF-α)水平。结果慢性阻塞性肺疾病患者急性发作期 ,IL-8及TNF-α水平分别为 ( 12 8.3± 44 .2 ) μg/L,( 164 9± 5 2 6) ng/L。稳定期 IL-8及 TNF-α水平分别为 ( 5 7.3± 3 3 .2 ) μg/L,( 4 2 6±2 0 0 ) ng/L ;IL-8及 TNF-α与 1秒钟用力呼气容积占预计值百分比 ( FEV1 占预计值 % ) ;1秒钟用力呼气容积 /用力肺活量 ( F EV1 /F Vc)呈显著相关。结论 IL-8、TNF -α共同参与 COPD气道炎症的形成 ,并与气道阻塞的程度密切相关。
Objective To investigate the role of IL-8 and TNF-α in the pathogenesis of chronic obstructive pulmonary disease (COPD). Methods Thirty patients with acute exacerbation of COPD and stable sputum were collected. The levels of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in sputum were measured by ELISA. Results The levels of IL-8 and TNF-α were (12 8.3 ± 44.2) μg / L and (164 9 ± 526) ng / L respectively in patients with chronic obstructive pulmonary disease. The levels of IL-8 and TNF-α in stable phase were (5 7.3 ± 3 3 .2) μg / L and (42 6 ± 2 0 0) ng / L, respectively. Exhaled volume accounted for the predicted percentage (FEV1 accounted for the predicted value); 1 second forced expiratory volume / forced vital capacity (F EV1 / F Vc) was significantly correlated. Conclusion IL-8 and TNF-α are involved in the formation of airway inflammation in COPD and are closely related to the degree of airway obstruction.