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目的探讨苦楝子多糖的提取工艺及体外抗氧活性。方法对影响苦楝子多糖提取的温度、时间和乙醇体积分数进行L9(34)正交试验优化,并采用超氧阴离子自由基(O2÷)和1,1-二苯基苦基苯肼(DPPH)自由基的反应体系,以维生素C为对比,研究该多糖的抗氧清除作用。结果提取时间显著影响多糖得率,最佳工艺条件为提取温度85℃、醇沉体积分数70%,时间2h。在此条件下,当料液比为1:1,提取1次时,多糖得率可达5.89%。苦楝子多糖对O2÷和DPPH具有较好的清除能力,对O2÷的EC50为2.21mg/mL;当苦楝子多糖质量浓度为5.02mg/mL时,其对DPPH的抑制率可超过50%。结论由最佳提取工艺获得的苦楝子多糖具有明显的抗氧化活性。
Objective To investigate the extraction of polysaccharides from Azadirachta indica and its antioxidant activity in vitro. Methods The temperature, time and volume fraction of ethanol extract from Melia azedarach were optimized by L9 (34) orthogonal test. The superoxide anion radical (O2 ÷) and 1,1-diphenyl picrylhydrazide ) Free radical reaction system, with vitamin C as contrast, to study the polysaccharide antioxidant scavenging effect. Results The extraction time significantly affected the yield of polysaccharides. The optimum conditions were extraction temperature 85 ℃, alcohol precipitation volume fraction 70% and time 2h. Under this condition, the yield of polysaccharide reached 5.89% when the ratio of solid to liquid was 1: 1 and extracted once. Artemisia polysaccharide had good scavenging ability on O2 ÷ and DPPH with an EC50 of 2.21 mg / mL for O2 ÷. When the concentration of Melia toosendanicum polysaccharide was 5.02 mg / mL, the DPPH inhibition rate could exceed 50%. Conclusion The results showed that the polysaccharides from Melia azedarach had the best antioxidant activity.