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目的:通过观察补阳还五汤调节高血压前期大鼠肾脏TGF-β_1/Smads通路信号分子的表达作用,阐释补阳还五汤调节高血压前期大鼠肾脏肌成纤维细胞异常转化,延缓肾脏病理损伤的作用。方法:将20只雄性盐抵抗大鼠(dahl salt resistant rat,SS)随机分为正常对照组,中药对照组,20只雄性dahl盐敏感大鼠(dahl salt sensitive rat,DS)随机分为模型组和中药治疗组,每组10只。高盐饲料喂养12 d后,中药对照组和中药治疗组予以补阳还五汤灌胃治疗,每日1次,共治疗5周。治疗结束后取大鼠左肾组织检测TGF-β_1Smad2 Smad7及α-SMA的mRNA表达和P-Smad2蛋白表达。结果:与正常组比较,模型组TGF-β_1Smad2和α-SMA的mRNA表达升高(P<0.05),Smad7mRNA表达降低(P<0.05),P-Smad2蛋白表达升高(P<0.05);补阳还五汤治疗后,与模型组比较中药治疗组TGF-β_1Smad2和α-SMA的mRNA表达降低(P<0.05);Smad7mRNA表达升高(P<0.05),P-Smad2蛋白表达降低(P<0.05)。结论:补阳还五汤可能是通过调节肾脏TGF-β_1/Smads通路相关信号分子的表达抑制肌成纤维细胞异常转化,延缓肾脏病理损伤。
OBJECTIVE: To observe the effect of Buyang Huanwu Decoction (TGF-β_1) on the expression of TGF-β_1 / Smads signaling pathway in prehypertensive rat kidney and to elucidate the effect of Buyang Huanwu decoction on the abnormal transformation of rat renal myofibroblasts in prehypertensive rats, The role of pathological damage. Methods: Twenty dahl salt resistant rats (SS) were randomly divided into normal control group, control group and dahl salt sensitive rat (DS). The rats were randomly divided into model group And traditional Chinese medicine treatment group, 10 in each group. After 12 days of feeding high-salt diet, the traditional Chinese medicine control group and traditional Chinese medicine treatment group were treated with Buyang Huanwu Decoction intragastrically once daily for 5 weeks. At the end of treatment, the left kidney tissue of rats was taken out to detect the mRNA expression of TGF-β 1 Smad7 and α-SMA and the expression of P-Smad2 protein. Results: Compared with the normal group, the mRNA expression of Smad7 and TGF-β1 Smad2 increased (P <0.05), the Smad7 mRNA decreased (P <0.05) and the expression of P-Smad2 increased Compared with the model group, the mRNA expression of TGF-β 1 Smad2 and α-SMA decreased (P <0.05), the expression of Smad7 mRNA increased (P <0.05) and the expression of P-Smad2 decreased 0.05). Conclusion: Buyang Huanwu Decoction may inhibit the abnormal transformation of myofibroblasts by regulating the expression of TGF-β1 / Smads pathway-related signaling molecules and delay the pathological changes of renal pathology.