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目的:探讨周期性张应力作用下人牙周膜成纤维细胞(HPDLF)转化生长因子β(1TGF-β1)对其细胞增殖的作用,及对其I型胶原基因表达的作用和影响。方法:在成功构建人牙周膜成纤维细胞体外培养力学刺激模型的基础上,利用多通道细胞牵张应力加载系统,对细胞分别施加2、6、12与24 h的周期性张应力,以不加力组为对照组,观察各组细胞形态变化,利用细胞计数试剂8检测细胞增殖活性,并利用ELISA试剂盒检测加力后各组TGF-β1的表达,并对加力12 h组加入TGF-β1抑制剂SB431542,利用RT-PCR检测技术检测对I型胶原基因表达的影响。结果:与对照组比较,加力2 h细胞增殖稍降低,6 h增殖活性增强,12 h达到峰值,24 h增殖活性明显受到抑制;TGF-β1的表达与细胞增殖成正相关;TGF-β1受到抑制后细胞增殖受到影响,I型胶原的表达也受到影响。结论:人牙周膜成纤维细胞的增殖在一定时间的周期性张应力作用下先增加然后再抑制,其中TGF-β1参与细胞增殖,并且TGF-β1对人牙周膜成纤维细胞I型胶原的表达起促进作用。
OBJECTIVE: To investigate the effect of transforming growth factor-β (TGF-β1) on the proliferation of human periodontal ligament fibroblasts (HPDLF) under cyclic tension stress and its effect on the gene expression of type I collagen. Methods: Based on the successful mechanical stimulation model of human periodontal ligament fibroblasts cultured in vitro and using the multi-channel cell stretch stress loading system, the cells were exposed to cyclic tensile stresses of 2, 6, 12 and 24 h respectively The rats in each group were sacrificed and the rats in each group were sacrificed. The morphological changes of each group were observed. The cell proliferation activity was detected by the cell counting reagent 8, and the expression of TGF-β1 in each group was detected by ELISA kit. TGF-β1 inhibitor SB431542, using RT-PCR detection of type I collagen gene expression. Results: Compared with the control group, the proliferation of cells at 2 h decreased slightly, the proliferation activity increased at 6 h, reached the peak at 12 h, and was significantly inhibited at 24 h. The expression of TGF-β1 was positively correlated with cell proliferation. TGF- Inhibition of cell proliferation is affected, type I collagen expression is also affected. CONCLUSION: The proliferation of human periodontal ligament fibroblasts firstly increases and then decreases under the action of cyclic tensile stress for a certain period of time. TGF-β1 is involved in cell proliferation. TGF-β1 can inhibit the proliferation of human periodontal ligament fibroblasts type I collagen The expression of the role of promotion.