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目的采用HPLC法测定中药材肿节风中黄酮类成分落新妇苷及柚皮苷的量。方法采用Diamonsil C18色谱柱(200 mm×4.6 mm,5μm);以乙腈(A)-0.1%磷酸水溶液(B)水溶液为流动相,采用梯度洗脱:0~10 min,A-B(10∶90);10~25 min,A-B(10∶90)→A-B(15∶85):25~35 min,A-B(15∶85)→A-B(20∶80)→B保留15min;体积流量采用程序控制0~10 min,0.5 mL/min;10~25 min,0.5→0.75 mL/min;25~35 min,0.7→0.8 mL/min;保留15 min;检测波长为290 nm;柱温:30℃。结果落新妇苷及柚皮苷的平均回收率分别为97.3%、95.6%,RSD分别为2.2%、2.3%;线性范围分别为0.028~0.27μg(r=0.999 6),0.030~0.29μg(r=0.999 5)。结论该方法简便、灵敏、快速、准确、重现性好,为肿节风药材的质量控制及合理开发利用提供了科学依据。
OBJECTIVE To determine the amount of astilbin and naringin flavonoids in the swollen section of Chinese herbal medicines by HPLC. Methods Diamonsil C18 column (200 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) -0.1% phosphoric acid solution (B) with gradient elution: 0-10 min, (15:85): 25 ~ 35 min, AB (15:85) → AB (20:80) → B for 15 min. The volume flow rate was controlled by the program 0 ~ 10 min at 0.5 mL / min; 10-25 min at 0.5 → 0.75 mL / min; 25-35 min at 0.7 → 0.8 mL / min; retention at 15 min; detection wavelength at 290 nm; Results The average recoveries of astilbin and naringin were 97.3% and 95.6%, respectively, with RSDs of 2.2% and 2.3%, respectively. The linear range was 0.028-0.27μg (r = 0.999 6) and 0.030-0.29μg = 0.999 5). Conclusion The method is simple, sensitive, rapid, accurate and reproducible. It provides a scientific basis for the quality control and rational development and utilization of the herbal medicine for the treatment of rheumatoid arthritis.