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为了比较不同提取方法对中药淫羊藿总黄酮含量的影响,试验采用水煎、超声波和回流法进行总黄酮的提取,采用紫外分光光度法测定其含量。结果表明:回流组和超声3组总黄酮含量极显著高于超声2组、超声1组和水煎组(P<0.01),超声2组总黄酮含量极显著高于超声1组和水煎组(P<0.01),超声1组总黄酮含量极显著高于水煎组(P<0.01)。说明采用回流法(用80%乙醇回流)提取淫羊藿总黄酮的提取率最高,水煎法提取率最低。
In order to compare the effects of different extraction methods on the total flavonoids content of Herba Epimedii, the total flavonoids were extracted by decoction, ultrasonic wave and reflux method. The content of total flavonoids was determined by UV spectrophotometry. The results showed that the content of total flavonoids in reflux group and ultrasound group was significantly higher than those in ultrasound group 2, ultrasound group 1 and decoction group (P <0.01). The content of total flavonoids in ultrasound group 2 was significantly higher than that in ultrasound group 1 and decoction group (P <0.01). The content of total flavonoids in ultrasound 1 group was significantly higher than that in decoction group (P <0.01). It shows that the extraction rate of total flavonoids extracted from Herba Epimedii by reflux method (using 80% ethanol reflux) is the highest, and the extraction rate is the lowest.