近年来,随着茎椰菜贮运技术得以解决,上海每年都有一定数量出口,但目前生产用种仍是国外进口的F_1种子,为了改变这一状况,迫切需要培育出我国自己的品种。在育种过程中,我们对茎椰菜的试管繁殖技术作了研究,并对如何应用组织培养技术来加快育种进程作了初步探索。组织培养技术本试验除了所用的诱导花粉愈伤组织培养基(B_5+2,4—D 2mg/1+KT 1mg/1)外,组 In recent years, with the solution of storage and transportation technology of broccoli, Shanghai has a certain amount of export every year. However, the current production of seeds is still F_1 imported from abroad. In order to change this situation, it is urgent to cultivate our own varieties. In the process of breeding, we made a study on tube propagation technology of broccoli, and made preliminary exploration on how to apply tissue culture technology to speed up the breeding process. Tissue Culture Techniques In this experiment, except for the induced pollen callus medium (B 5 + 2,4-D 2 mg / 1 + KT 1 mg / 1)