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GDP、GTP和GTPγS激活北京鸭红细胞膜上的磷酯酶C,在10~(-7)～10~(-4)mol/L的范围内,使磷酸肌醇产量增加。其中GTPγS作用最强,分别使IP_2和IP_3增强550％和580％。GTP与GDP的效力相近,使IP_2增加140％和150％;IP_3增加230％和330％。A-嘌呤能受体激动剂6-N-cyclohe-xyladenosine(CHA)对抗ATP通过P-嘌呤能受体激活的磷酯酶C,使其活性下降60％。CHA的这种作用可被其特异拮抗剂8-cyclopentyl-1,3-dimethylxanthine对抗。以上结果表明鸭红细胞是研究受体、G-蛋白及磷酯酶C偶联的良好模型。表明不同嘌呤能受体在调节磷酯酶C中的相反作用。
GDP, GTP and GTPγS activated phospholipase C in Beijing duck erythrocyte membrane, and increased phosphoinositide production in the range of 10 ~ (-7) ~ 10 ~ (-4) mol / L. Among them, GTPγS had the strongest effect, which enhanced IP_2 and IP_3 by 550% and 580% respectively. Similar effects of GTP and GDP have led to an increase of IP_2 by 140% and 150%; IP_3 by 230% and 330% respectively. A-purinergic receptor agonist 6-N-cyclohe-xyladenosine (CHA) counteracts ATP activity by P-purinergic receptor phospholipase C by a factor of 60%. This effect of CHA can be countered by its specific antagonist 8-cyclopentyl-1,3-dimethylxanthine. The above results indicate that duck erythrocytes are a good model to study the coupling of receptor, G-protein and phospholipase C. Indicating the opposite effect of different purinergic receptors in the regulation of phospholipase C.