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目的:比较在腹腔注射相同剂量阿霉素(12 mg/kg)下,不同给药方案构建扩张型心肌病大鼠模型的生存率。创新点:首次应用腹腔注射阿霉素1 mg/kg,一周两次的方法构建扩张型心肌病大鼠模型,并与常规的方法进行生存率的比较。方法:将150只SD雄鼠分为对照组(腹腔注射生理盐水)、Dox 1组(腹腔注射阿霉素1 mg/kg一周两次,共六周)和Dox 2组(腹腔注射阿霉素2 mg/kg一周一次,共六周)。观察及比较各组大鼠的体重、生存率、心腔大小、pro-BNP、CRP、CREA、AST和Caspase-3 mR NA水平。观察各组大鼠心肌病理变化,同时进行~(18)FDG-PET心肌代谢显像。结论:本实验中结果显示,Dox 1组和Dox 2组在pro-BNP、Caspase-3 mR NA水平、心肌病理变化及~(18)FDG-PET心肌代谢显像上均没有明显差异;但Dox 1组的生存率是78%,而Dox 2组的生存率是52%;同时,Dox 2组大鼠的血清CREA、AST和CRP水平比Dox 1组明显升高。综上所述,腹腔注射阿霉素1 mg/kg一周两次的方法在成功构建扩张型心肌病大鼠模型的同时大大提高了存活率。
OBJECTIVE: To compare the survival rate of rat model of dilated cardiomyopathy constructed under different dosing regimens with the same dosage of doxorubicin (12 mg / kg). Innovation: The first application of intraperitoneal injection of doxorubicin 1 mg / kg, twice a week to build dilated cardiomyopathy rat model and compared with the conventional method of survival. Methods: 150 SD male rats were divided into control group (intraperitoneal injection of normal saline), Dox 1 group (intraperitoneal injection of doxorubicin 1 mg / kg twice a week for six weeks) and Dox 2 group (intraperitoneal injection of doxorubicin 2 mg / kg once a week for a total of six weeks). The body weight, survival rate, heart size, pro-BNP, CRP, CREA, AST and Caspase-3 mR NA were observed and compared in each group. The pathological changes of myocardium in each group were observed, and ~ (18) FDG-PET myocardial metabolic imaging was performed at the same time. CONCLUSIONS: The results in this study showed no significant differences in pro-BNP, Caspase-3 mR NA levels, myocardial pathological changes, and 18G FDG-PET myocardial metabolic imaging between Dox 1 and Dox 2 groups; however, Dox The survival rate of group 1 was 78%, while the survival rate of group Dox 2 was 52%. Meanwhile, the levels of CREA, AST and CRP in group Dox 2 were significantly higher than those in group Dox 1. In summary, intraperitoneal injection of doxorubicin 1 mg / kg once a week twice a week in the successful construction of rat model of dilated cardiomyopathy while greatly improving the survival rate.