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对已建立的水稻耐光抑制种质资源的简易批量筛选技术,进行了可行性的论证和原理探讨、实验证明:将离体叶片放入水中经高光强处理,是一种人上光抑制条件.在这种条件下,品种间对光抑制的敏感性有显著差异.经测定耐光抑制的品种光合速率、PSII活性、Q_B蛋白表现相对稳定,这与内源保护物质SOD诱导活性高,有毒的活性氧积累少有关.因此这类品种在简易筛选条件下叶绿素降解缓慢,可见光氧化(叶色)伤害较轻,而对光抑制敏感的品种则在生理上有相反的表现.用这种简易技术对75份水稻种质资源进行筛选,经初步鉴定:粳稻多为耐性品种;籼稻多为敏感性品种,但籼稻中也具有耐性强的品种,如美国Pecos,Bellemont;菲律宾IR38,IR43,IR52;斯里兰卡BG90-2在抽穗期表现耐光抑制特性.
The feasibility of the simple batch screening technology for rice light-resistant germplasm resources was discussed and the principle was discussed. The experiment proves that putting the detached leaves into the water and treating with high light intensity is a kind of man-smelling inhibition condition. Under these conditions, there was a significant difference in susceptibility to photoinhibition among varieties.The photosynthetic rate, PSII activity and Q-B protein performance of the tested varieties were relatively stable, which was consistent with the high activity of SOD induced by endogenous protective substances and toxic reactive oxygen species Accumulation of less relevant.Therefore, these varieties in the simple screening conditions chlorophyll degradation slow, visible oxidation (leaf color) damage lighter, while sensitive to photoinhibition of the species are physiologically opposite performance with this simple technique of 75 The rice germplasm resources were screened. The preliminary identification was that mostly japonica rice was tolerant; indica rice was mostly sensitive, but indica rice also had strong tolerance varieties such as Pecos and Bellemont in the United States; Philippines IR38, IR43 and IR52; Sri Lanka BG90 -2 showed photosynthetic light-fasting inhibition at heading.