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目的 确认一对细胞来源相同、转移能力不同的人肺腺癌细胞系 AGZY83- a和 Anip- 973中3号染色体短臂的分子细胞遗传学差异。方法 采用 3p涂染探针对两细胞系进行 G显带后荧光原位杂交。结果 两细胞系共同存在标记染色体 der(3;5 ) (3pter→ 3p10∷ 5 q10→ 5 qter)和 der(1) t(1;12 ;3)(3pter→ 3q12∷ 12 q2 4→ 12 q15∷ 1p2 2→ 1qter)。不同的是 ,低转移细胞系 AGZY83- a中存在 1条正常的 3号染色体 ;而在高转移细胞系 Anip973中 ,存在 1条涉及 3号染色体断裂重排的标记染色体 +(?∷ 3p2 4→3qter) ,断裂点发生于转移相关基因 RAB5 A的染色体区域。结论 高转移肺腺癌细胞系 Anip973中 3号染色体上 RAB5 A基因区 3p2 4的断裂重排与该基因的高表达一致。
Objective To confirm the molecular cytogenetic differences of the short arm of chromosome 3 in human lung adenocarcinoma cell lines AGZY83-a and Anip-973 with the same cell origin and different metastatic potential. Methods G-banding and post-fluorescence in situ hybridization were performed on the two cell lines using 3p-coated probes. Results Both cell lines co-existed the marker chromosome der (3; 5) (3pter → 3p10 :: 5 q10 → 5 qter) and der (1) t (1; 12; 3) 1p2 2 → 1qter). The difference is that there is one normal chromosome 3 in AGZY83-a cell line with low metastasis. In the high-metastatic cell line Anip973, there is one marker chromosome which is involved in the rearrangement of chromosome 3 (+ 3p2 4 → 3qter). The break point occurred in the chromosomal region of RAB5 A gene. Conclusion The rearrangement of 3p24 in RAB5 A gene region on chromosome 3 of Anip973 cell line is consistent with the high expression of this gene.