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采用室内生物测定方法,研究了除草剂解毒剂ND2144(N-二氯乙酰基-2-甲基-1-氧杂-4-氮杂-螺[4.4]壬烷)减轻除草剂乙草胺残留对玉米产生的药害,初步探讨其解毒机理。结果表明:随土壤中乙草胺残留浓度的增加,玉米的生长受到抑制。采用不同浓度ND2144浸种处理后,均可在一定程度上减轻乙草胺残留对玉米产生的药害。当土壤中乙草胺的残留浓度为10～20mg·kg-1,ND2144的浸种浓度为8mg·kg-1时解毒效果最好,玉米株高、株鲜重的恢复率可达对照的102.56%和97.19%,幼苗中的谷胱甘肽(glutathione,GSH)含量和乙酰乳酸合成酶(acetolactate synthase,ALS)活性分别达到对照的108.1%和142.9%。
The herbicide antidote ND2144 (N-dichloroacetyl-2-methyl-1-oxa-4-aza-spiro [4.4] nonane) was used to reduce the herbicide acetochlor residue using indoor bioassay The harm to maize, to explore the mechanism of detoxification. The results showed that the growth of maize was inhibited with the increase of acetochlor residual concentration in the soil. Soaking seedlings with different concentrations of ND2144 could alleviate the phytotoxicity of acetochlor to corn to a certain extent. When the residual concentration of acetochlor in the soil was 10 ~ 20 mg · kg-1, the effect of detoxification was the best when the concentration of ND2144 was 8 mg · kg-1, and the recovery rate of plant height and fresh weight of maize reached 102.56% And 97.19% respectively. The contents of glutathione (GSH) and acetolactate synthase (ALS) in seedlings reached 108.1% and 142.9% of the control, respectively.