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将新建立的检测血清中抗丙型肝炎病毒（ＨＣＶ）总抗体的双抗原夹心ＥＬＩＳＡ（夹心法）与普遍采用的检测抗ＨＣＶ－ＩｇＧ的间接ＥＬＩＳＡ（ＩｇＧ间接法）及检测抗ＨＣＶ－ＩｇＭ的间接ＥＬＩＳＡ（ＩｇＭ间接法）进行比较研究。检测１５份ＩｇＭ间接法阳性、ＩｇＧ间接法阴性（ＩｇＭ单阳）血清及２０份ＩｇＧ间接法阳性、ＩｇＭ间接法阴性（ＩｇＧ单阳）血清，夹心法均为阳性，这３５份血清经ＰＣＲ检测ＨＣＶＲＮＡ均阳性。夹心法可同时检出ＩｇＧ和ＩｇＭ抗体，检出率高于后两者。检测２０份ＨＣＶＲＮＡ阴性血清，３种方法均阴性。用生物制品检定所检测ＨＣＶ－ＩｇＧ抗体的质控血清为标准品，夹心法和ＩｇＧ间接法均可达到检测标准
The newly established double antigen sandwich ELISA (sandwich method) for detecting the total anti-hepatitis C virus (HCV) antibody in serum and the commonly used indirect ELISA (IgG indirect method) for detecting anti-HCV-IgG and the method for detecting anti-HCV- Indirect ELISA (IgM indirect method) for comparative study. The 15 indirect IgM positive, IgG indirect negative (IgM single positive) sera and 20 IgG indirect positive, IgM indirect negative (IgG single positive) serum, sandwich method were positive, the 35 serum detected by PCR HCVRNA are positive. Sandwich method can detect both IgG and IgM antibodies, the detection rate is higher than the latter two. Twenty HCVRNA-negative sera were tested negative for all three methods. The quality control serum of the HCV-IgG antibody detected by the biological product test is a standard sample, and the sandwich standard method and the IgG indirect method can both achieve the detection standard