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动物肝脏是合成各类载脂蛋白的主要部位。同时,肝脏的各类载脂蛋白都与心血管疾病有关.目前,对载脂蛋白的研究主要集中在其蛋白结构和胆固醇脂形成过程的几个方面。为了深入研究载脂蛋白基因作用的分子机制,我们从北京鸭肝中提取总mRNA,合成总cDNA,首次组建了鸭肝组织的总cDNA库。从北京鸭肝组织中提取总mRNA时是用oligod(T)纤维素柱层析纯化mRNA,经过
Animal liver is the main part of the synthesis of various types of apolipoprotein. At the same time, all kinds of liver apolipoproteins are related to cardiovascular diseases.At present, the research on apolipoproteins mainly focuses on several aspects of the protein structure and cholesterol lipid formation process. In order to further study the molecular mechanism of apolipoprotein gene function, we extracted total mRNA from Beijing duck liver and synthesized total cDNA. The total cDNA library of duck liver tissue was established for the first time. When extracting total mRNA from duck liver tissue in Beijing, mRNA was purified by oligod (T) cellulose column chromatography