羊水细胞培养是产前诊断染色体病和先天性代谢病的重要手段,成功而有效的分析取决于羊水细胞培养的质量。参考1983年Chettur提出的方法,我们从小牛脑垂体制备了成纤维细胞生长因子(Fibroblast growthFactor, FGF)粗制品①,对羊水细胞培养的全过程进行了观察和研究,发现FGF对羊水细胞生长有一定的促进作用。1984年我们又在羊水细胞培养中添加有机缓冲剂N-2hydroxyethyl-piperazine-N-2′-ethane-sul Amniotic fluid cell culture is an important means of prenatal diagnosis of chromosome disease and congenital metabolic disease, successful and effective analysis depends on the quality of amniotic fluid cell culture. According to the method proposed by Chettur in 1983, we prepared a crude product of fibroblast growth factor (FGF) from the calf pituitary, and observed and studied the whole process of amniotic fluid cell culture. We found that FGF has A certain role in promoting. In 1984 we again in the amniotic fluid cell culture added organic buffer N-2hydroxyethyl-piperazine-N-2’-ethane-sul