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目的采用HPLC测定三味蔷薇胶囊中的没食子酸。方法采用ZorbaxEclipseXDB-C18柱(150mm×3.9mm,5μm),流动相为乙腈-0.2%磷酸溶液(2∶98),柱温35℃,流速0.8mL·min-1,检测波长270nm。结果没食子酸3.306～66.12μg·mL-1与峰面积的线性关系良好(r=0.9996),平均回收率为98.38%(RSD=1.41%,n=9),精密度和重复性的RSD分别为1.15%、1.83%。结论所建方法简便、快速、灵敏、准确。
Objective To determine gallic acid in Sanwei Rosa capsules by HPLC. Methods The Zorbax Eclipse XDB-C18 column (150 mm × 3.9 mm, 5 μm) was used. The mobile phase was acetonitrile-0.2% phosphoric acid solution (2:98). The column temperature was 35 ℃ and the flow rate was 0.8 mL · min- Results The linear relationship between the concentration of gallic acid and peak area was 3.306 ~ 66.12μg · mL-1 (r = 0.9996), the average recovery was 98.38% (RSD = 1.41%, n = 9). The RSD of precision and repeatability were 1.15%, 1.83%. Conclusion The method is simple, rapid, sensitive and accurate.