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目的 了解TT病毒 (Transfusiontransmittedvirus,TTV)在我国的流行情况 ,研究我国TTV株序列特点。方法 采用半巢式聚合酶链反应 (PCR)扩增TTVDNA ,PCR阳性扩增产物直接测序。结果 我国 7例TTVDNA株部分序列与日本株相比 ,核苷酸及氨基酸同源性分别为 6 4 7%～ 98 4%及 6 2 7%～ 96 4% ;7株间的核苷酸及氨基酸同源性分别为 6 3 5 %～ 98 4%及 6 0 2 %～ 96 4% ,分别属于两种型及其中一型中的两种亚型。结论 我国存在TTV并可能存在多种TTV基因型
Objective To understand the prevalence of TTV in China and to study the characteristics of TTV strain in China. Methods TTVDNA was amplified by semi-nested polymerase chain reaction (PCR), and the positive PCR products were directly sequenced. Results The nucleotide and amino acid homology of partial sequences of 7 TTVDNA strains in China was between 64.7% ~ 98.4% and 62.7% ~ 96.4%, respectively. Compared with the Japanese strains, The amino acid homologies were between 6 3 5% and 98 4% and between 6 0 2% and 96 4% respectively, belonging to two subtypes of two types and one type respectively. Conclusion There are many TTV genotypes in China