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目的 :探讨异种微囊化兔坐骨神经组织对大鼠脊髓损伤保护作用的分子机制。方法 :⑴取兔坐骨神经剪碎 ,加胰蛋白酶消化 ,滤液用 1.5 %海藻酸钠混匀 ,喷入Bacl2液混匀、收集含有神经细胞 /组织的微囊 ;⑵A正常对照组 5只 ;B空囊组 3 0只 ;C微囊化兔坐骨神经组织移植组 3 0只 ;⑶动物模型的建立 :动物麻醉后 ,咬除T9～ 11胸椎棘突椎板 ,暴露脊髓 ,左半侧脊髓洞切损伤 ;B组洞腔植入空囊 ;C组植入等量的微囊化坐骨神经组织 ,各组于术后不同时间各取 5只SD鼠 ,取损伤区脊髓组织切片。A组取相应处脊髓 ;⑷bcl-2免疫组化染色 ;⑸TUNEL染色。结果 :TUNEL检测见胞核呈棕黄颗粒状凋亡细胞 ;微囊化兔坐骨神经组织移植组出现大量bcl-2蛋白阳性细胞。结论 :微囊化兔坐骨神经组能对抗脊髓损伤后神经元凋亡 ,促进bcl-2蛋白表达 ,对脊髓继发性损伤提供保护作用
Objective: To explore the molecular mechanism of the protective effect of heterogeneous microencapsulated rabbit sciatic nerve tissue on spinal cord injury in rats. Methods: (1) Rabbit sciatic nerve was cut into pieces and trypsinized. The filtrate was mixed with 1.5% sodium alginate and sprayed into Bacl2 solution to mix the microcapsules containing nerve cells / tissues. (2) A normal control group 30 in C group and 30 in C sciatic nerve tissue transplantation group. ⑶ Establishment of animal model: After anesthesia, the animals were anesthetized and bite except for T9 ~ 11 thoracic vertebral lamina, exposing the spinal cord and the left hemisphere. ; Group B was implanted with empty cavities; Group C was implanted with the same amount of microencapsulated sciatic nerve tissue. Five SD rats were taken at different time points after operation in each group. Spinal cord tissue sections were taken from the injured area. A group of corresponding spinal cord; ⑷bcl-2 immunohistochemical staining; ⑸TUNEL staining. Results: Apoptotic cells with brown granules and yellow granules were found in TUNEL assay. A large number of bcl-2 positive cells appeared in the microencapsulated rabbit sciatic nerve tissue transplantation group. CONCLUSION: The microencapsulated rabbit sciatic nerve group can antagonize the neuronal apoptosis after spinal cord injury, promote the expression of bcl-2 protein and provide a protective effect on secondary spinal cord injury