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对进境旅客携带芒果进行检疫时,在一枚芒果上发现了25头实蝇科幼虫,化蛹后显示存在两种形态特征差异较大的蛹,其中类型I的蛹10头,类型II的蛹15头。类型I中的5个蛹成功羽化为成虫,经形态鉴定为非洲芒果实蝇。类型II的蛹未成功羽化。由于对蛹的鉴定尚有困难,分别提取了两种类型蛹的线粒体DNA,分别扩增了COI、COII、COIII基因,测序结果与Gen Bank数据库进行序列比对分析。结果显示,类型I蛹COI、COII、COIII基因序列分别与非洲芒果实蝇相应基因的序列相似性达到99%以上,与形态学鉴定结果相同。类型II蛹COI基因序列与入侵果实蝇的基因序列相似性达到100%。综上结果,类型I幼虫为非洲芒果实蝇,而类型II为入侵果实蝇。在同一枚芒果上发现两种不同的检疫性实蝇,比较少见,增加了水果检疫的难度,在实际工作中应给予高度重视。
When quarantine carried passengers carrying mangoes, 25 muscidae larvae were found on one mango. After pupation, there were two pupae with different morphological characteristics, of which 10 were type I pupae, type II Pupa 15 head. Five pupae in type I were successfully induced to adult and morphologically identified as African mango fruit fly. Type II pupae did not succumb to emergence. Because of the difficulty of identification of pupae, mitochondrial DNA of two types of pupae were extracted respectively, and COI, COII and COIII genes were amplified respectively. The sequencing results were compared with that of Gen Bank database. The results showed that the sequence similarity of the COI, COII and COIII genes of pupae of type I were more than 99% with the corresponding gene of African mango fruit fly, which was the same as that of morphological identification. Genotype II pupae COI gene sequence and invasive fruit fly gene sequence similarity of 100%. In summary, type I larvae were African mango fruit flies, while type II was invasive fruit flies. Two different quarantine fruit flies found on the same mango are relatively rare, which increases the difficulty of quarantine fruit and should be given high priority in practical work.