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就转cry1Ac+CpTI双基因抗虫水稻不同生育期对二化螟Chilosuppressalis和大螟Sesamiainferens的室内致死特性及田间螟虫的构成进行了研究。室内测定结果表明,不同生育期转基因水稻对二化螟、大螟都表现明显的致死效应,但水稻生长后期的致死效果降低。转基因水稻对大螟的致死效应显著弱于对二化螟的,其中,二化螟除在齐穗期和成熟期有少量幼虫(0.5%~6.4%)存活到第4天外,其余均在第4天死亡;大螟在两种转基因水稻上的存活率高于二化螟,且少量个体(<1.6%)还能化蛹、羽化,但化蛹率和羽化率均明显低于在非转基因对照上的。早、晚两季水稻的田间调查结果表明,转基因水稻上两种螟虫虫口数量均显著低于相应的非转基因对照品种,转基因水稻上二化螟虫口减退率>99%;大螟虫口减退率相对较低,早、晚稻上有所不同,其中早稻>93%,晚稻仅44%~64%。转基因水稻上残存螟虫中,大螟所占比例明显上升,推测转基因水稻对两种螟虫致死效应差异可能是其主要原因。
The indoor lethality of Chilo suppressalis and Sesamia inferees and the composition of field stem borers were investigated at different growth stages of transgenic rice plants with cry1Ac + CpTI double resistance. Laboratory results showed that transgenic rice at different growth stages showed obvious lethal effect on the rice stem borer, Sesamia inferens, but the lethal effect at the late rice growth stage was reduced. The lethal effect of transgenic rice on Sesamia inferens was weaker than that of Sesamia inferens. Among them, Sesamia inferens except for a few larvae (0.5% ~ 6.4%) at heading and maturity survived to the 4th day, 4 days. The survival rate of Sesamia in both transgenic rice was higher than that of Chilo suppressalis, and a small number of individuals (<1.6%) were able to pupae and emergence, but the pupation rate and emergence rate were significantly lower than those in non-transgenic On the control. The field survey results of rice in the early and late seasons showed that the population of the two borer borers in transgenic rice was significantly lower than that of the corresponding non-transgenic control varieties, and the rate of decline was> 99% in the transgenic rice stem borers. The reduction rate of the stem borer was relatively Lower, early and late rice are different, of which> 93% of early rice, late rice only 44% to 64%. The proportion of Sesamia inferens on transgenic rice was significantly increased. It is speculated that the lethal effect of transgenic rice on the two stem borers may be the main reason.