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目的研究赖氨大黄酸(rhein lysinate,RHL)对快速老化小鼠(senescence accelerated mouse prone 10,SAMP 10)肾组织Ⅰ型胶原α1链(COL1A1)、Ⅲ型胶原α1链(COL3A1)和Ⅳ型胶原α1链(COL4A1)蛋白表达的影响。方法选取7月龄SAMP 10小鼠18只,并根据随机数字表分为空白对照组、低剂量RHL组(25 mg/kg)和高剂量RHL组(50 mg/kg),每组6只;另选抗快速老化小鼠(senescence accelerated mouse resistance 1,SAMR 1)6只作为青年对照,给药时间6个月。采用HE染色观察肾脏组织病理改变,Masson染色观察肾脏组织纤维化改变,Western blot方法检测肾组织COL1A1、COL3A1、COL4A1和NF-κB蛋白的表达。结果与SAMP 10空白对照组相比,25和50 mg/kg RHL组治疗后SAMP 10小鼠精神状态、活动度、毛色等方面较好;肾脏指数升高。HE染色显示SAMP 10空白对照组小鼠肾组织有节段性肾小球萎缩、硬化和明显的间质纤维化,SAMR 1组和RHL组小鼠则无肾小球萎缩、硬化和明显的间质纤维化。RHL能够抑制SAMP 10小鼠衰老过程中出现的肾组织COL1A1、COL3A1和NF-κB蛋白过度表达(P<0.05)。结论 RHL可通过抑制COL1A1、COL3A1和NF-κB蛋白过度表达,减轻衰老过程中SAMP 10小鼠肾组织纤维化程度,发挥肾脏保护作用。
Objective To investigate the effect of rhein lysinate (RHL) on the expression of collagen type 1 α1 chain (COL1A1), collagen type Ⅲ chain (COL3A1) and type Ⅳ collagen in senescence accelerated mouse prone 10 (SAMP 10) α1 chain (COL4A1) protein expression. Methods 18 SAMP 10 mice of 7 months old were selected and divided into blank control group, low dose RHL group (50 mg / kg) and high dose RHL group (6 mg / kg) according to random number table. Six senescence accelerated mouse resistance 1 (SAMR 1) mice were selected as young controls for 6 months. The pathological changes of kidney were observed by HE staining. The changes of renal tissue fibrosis were observed by Masson staining. The expressions of COL1A1, COL3A1, COL4A1 and NF-κB in renal tissues were detected by Western blot. Results Compared with the SAMP 10 blank control group, the SAMP 10 mice in the 25 and 50 mg / kg RHL group showed better mental status, activity and hair color, and the kidney index increased. HE staining showed segmental glomerular atrophy, sclerosis and obvious interstitial fibrosis in the kidney of SAMP 10 blank control mice. There was no glomerular atrophy in the SAMR 1 and RHL mice, Fibrosis. RHL inhibited the over-expression of COL1A1, COL3A1 and NF-κB in renal tissues of SAMP 10 mice (P <0.05). Conclusion RHL can inhibit the expression of COL1A1, COL3A1 and NF-κB in renal tissue and reduce the renal fibrosis in SAMP 10 mice during aging.