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目的:分析骨碎补总黄酮对大鼠血管内皮细胞功能和血管形成的影响。方法:体外培养大鼠血管内皮细胞,用不同浓度(1,10,100mg/L)的骨碎补总黄酮干预后,MTT法检测细胞增殖,ELISA法检测内皮素(ET)和血管内皮生长因子(VEGF),Griess法检测NO的表达;利用去卵巢骨质疏松大鼠模型(36只,随机分为去卵巢组、假手术组和骨碎补治疗组),骨碎补总黄酮治疗3个月后,检测大鼠血清中ET,VEGF和NO的表达,骨密度和股骨远端微血管密度变化。结果:各浓度的骨碎补总黄酮均能促进血管内皮细胞的增殖,而中、高浓度促增殖效果较好;中、高浓度的骨碎补总黄酮在体内外均可以抑制ET,同时增强NO的表达;股骨远端血管计数结果显示骨碎补治疗组明显多于去卵巢组,并且与骨密度呈正相关;骨碎补总黄酮在体外可以增强内皮细胞VEGF的表达,但对去卵巢大鼠血清中VEGF的表达无影响。结论:骨碎补总黄酮可促进血管内皮细胞增殖,同时可以调节内皮细胞功能,促进血管形成,从而发挥抗骨质疏松作用。
OBJECTIVE: To analyze the effect of Rhizoma Drynariae flavonoids on the function and angiogenesis of vascular endothelial cells in rats. Methods: The vascular endothelial cells were cultured in vitro. The cells were treated with total flavonoids of different concentrations (1, 10, 100 mg / L) and MTT assay. The levels of endothelin (ET) and vascular endothelial growth factor VEGF). The expression of NO was detected by Griess method. Using the ovariectomized rat model (36, randomly divided into ovariectomized group, sham-operated group and rhubarb-treated group) Afterwards, the changes of ET, VEGF and NO, BMD and distal femur microvessel density were detected in the rat serum. Results: Total flavonoids of Rhizoma Drynariae could promote the proliferation of vascular endothelial cells, while the medium and high concentrations of Rhizoma Drynariae could promote the proliferation of Rhizoctonia solani. Both middle and high concentrations of Rhizoma Drynariae could inhibit ET both in vivo and in vitro NO expression; distal femoral vascular count results showed that the treatment group was significantly more than those in the ovariectomized ovariectomized group, and with a positive correlation between bone mineral density; total flavonoids drynariae in vitro can increase VEGF expression in endothelial cells, but large ovariectomized The expression of VEGF in rat serum had no effect. Conclusion: Total Flavonoids of Rhizoma Drynariae can promote the proliferation of vascular endothelial cells, regulate the function of endothelial cells and promote the formation of blood vessels, thereby exerting anti-osteoporosis effect.