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目的探究瘦素受体基因Gln223Arg多态性与上海某区儿童超重肥胖的关系及相关生化指标的相关性。方法采用PCR-RFLP对上海松江区六所小学7-11岁超重肥胖与正常体重儿童(各262名)的血样进行LEPR基因Gln223Arg多态性检测,logistic回归分析其与儿童超重肥胖的相关性,并分析了生化指标在超重肥胖组与正常组儿童以及不同基因型间的差异。结果超重肥胖组与对照组之间LEPR基因Gln223Arg AA频率与A等位基因频率均无统计学差异(P>0.05)。对其收缩压(SBP),舒张压(DBP),胆固醇(TC),甘油三酯(TG),血糖(GLU),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)进行t检验,得出SBP,DBP,TG,HDL-C和LDL-C在超重肥胖组与对照组中差异显著。在控制了混杂因素BMI等后,LEPR基因Gln223Arg各基因型组的生化指标差异分析表明基因型可能与LDL-C相关(P<0.05)。进一步两两比较结果表明LDL-C水平在基因型AA组比AG(P=0.019)与GG组(P=0.017)低,而AG与GG组之间的差异无统计学意义(P=0.517)。结论 LEPR基因Gln223Arg多态性可能与儿童超重肥胖的发生无关,而LDL-C(P<0.05)可能与LEPR基因Gln223Arg基因型有相关性。
Objective To investigate the relationship between the Gln223Arg polymorphism of leptin receptor gene and overweight and obesity in a district of Shanghai and the related biochemical markers. Methods PCR-RFLP was used to detect the polymorphism of LnpR gene Gln223Arg in blood samples of 7-11-year-old overweight and obese children (each 262) in six primary schools in Songjiang District of Shanghai. Logistic regression analysis was used to analyze the association with overweight and obesity in children. And analyzed the biochemical indicators in overweight and obesity group and normal group children and different genotypes differences. Results There was no significant difference in the frequencies of A allele and Gln223Arg between LEPR gene and control group (P> 0.05). SBP, DBP, TC, TG, GLU, HDL-C, LDL-C, C) t-test, drawn SBP, DBP, TG, HDL-C and LDL-C in overweight and obesity group and the control group significant difference. After controlling the confounding factors such as BMI, the difference of biochemical indexes between the genotypes of LnPLG and Gln223Arg genotypes indicated that genotypes may be related to LDL-C (P <0.05). Further comparisons showed that LDL-C levels were lower in genotype AA than in AG (P = 0.019) and GG (P = 0.017), but no significant difference was found between AG and GG (P = 0.517) . Conclusions Gln223Arg polymorphism of LEPR gene may not be associated with childhood overweight and obesity, whereas LDL-C (P <0.05) may be related to the genotype of LEPR Gln223Arg.