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目的探讨急性酒精性肝损伤与肝细胞凋亡的关系。方法将50只小鼠随机分成对照组10只和实验组40只。实验组每只小鼠按体重10 g/kg给47.5%酒精一次性灌胃。对照组每只小鼠按体重10 g/kg给生理盐水一次性灌胃。灌胃后分4、8、16和24 h分批随机取肝组织做光镜、TUNEL检测。结果实验组水样变性、细胞凋亡小鼠数达100%,实验组24 h时间段凋亡肝细胞核数占肝细胞核总数比是36.6%,24 h时间段片状坏死小鼠数达100%。对照组小鼠未见异常病变。结论急性酒精性肝损伤与肝细胞凋亡有明显的关系,且肝细胞凋亡随肝损伤的加重而增加。
Objective To investigate the relationship between acute alcoholic liver injury and hepatocyte apoptosis. Methods Fifty mice were randomly divided into control group (n = 10) and experimental group (n = 40). Each mouse in the experimental group was given a single gavage with 47.5% alcohol at a weight of 10 g / kg. Each mouse in the control group was given a single gavage with saline at a weight of 10 g / kg. After gavage, the liver tissues were randomly divided into light microscopy, TUNEL detection at 4, 8, 16 and 24 h. Results The number of apoptotic mice in experimental group was 100%. The number of apoptotic hepatocytes in experimental group at 24 h was 36.6% of the total number of hepatocytes, and the number of necrosis mice was 100% at 24 h. . Control mice showed no abnormal lesions. Conclusion Acute alcoholic liver injury has obvious relationship with hepatocyte apoptosis, and hepatocyte apoptosis increases with the increase of liver injury.