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小鼠白血病L 615是由津638病毒诱发的615系小鼠白血病的脾细胞悬液在同系成年小鼠移植而获得,它是一株可移植的网织细胞型白血病。经过408代的接种传代,生长特性相对稳定,发病率100%,无自发缓解,平均存活时间6.5天,细胞增殖动力学的研究表明,接种后第5天,L615白血细胞的增殖周期时间为13.8小时,3H-TdR连续标记24小时后,几乎所有白血细胞均被标记,生长比率近于1。L615白血细胞于局部接种后迅速通过血行向全身脏器播散,3小时后即可到达脾脏,各脏器的白血细胞浸润形式具有以血管为中心的特点。口服白血病脾细胞悬液在短期内也可使动物发病致死,发病原因,可能是活的白血细胞直接侵入。电子显微镜观察,发现白血细胞内含有较丰富的“池内A颗粒”。该模型对抗癌药物的敏感性比较广泛,尤其对抗代谢类及烷化剂类药物更为明显,因而,利用此模型筛选新的抗癌药物提供了较广泛的可能性。
Mouse leukemia L 615 is a transplanted reticulocyte leukemia obtained by transplanting the 615-derived mouse leukemia-derived spleen cell suspension induced by the Tsuguin-638 virus in a syngeneic adult mouse. After 408 generations of inoculation, the growth characteristics are relatively stable, the incidence rate of 100%, no spontaneous remission, the average survival time of 6.5 days, cell proliferation kinetics studies have shown that after 5 days of inoculation, L615 white blood cell proliferation cycle time was 13.8 Hour, almost all white blood cells were labeled after 3H-TdR was continuously labeled for 24 hours, with a growth rate of nearly 1. After local inoculation, L615 white blood cells spread rapidly to whole body organs through the blood line, and reached the spleen in 3 hours. The white blood cell infiltration pattern of various organs has the characteristics of blood vessel center. Oral leukemia spleen cell suspension in the short term can also cause animal disease and death, the etiology may be direct intrusion of live white blood cells. Electron microscopy, found that white blood cells contain more abundant “pool of A particles ”. The model is more sensitive to anti-cancer drugs, especially against the metabolism of metabolites and alkylating drugs more obvious, therefore, the use of this model screening of new anticancer drugs offers a wider range of possibilities.