Seasonal variation of biochemical components in clam(Saxidomus purpuratus Sowerby 1852) was investigated from March 2012 to February 2013 in relation to environmental condition of Sanggou Bay and the reproductive cycle of clam.According to the histological analysis,the reproductive cycle of S.purpuratus includes two distinctive phases:a total spent and inactive stage from November to January,and a gametogenesis stage,including ripeness and spawning,during the rest of the year.Gametes were generated at a low temperature(2.1℃) in February.Spawning took place once a year from June to October.The massive spawning occurred in August when the highest water temperature and chlorophyll a level could be observed.The key biochemical components(glycogen,protein and lipid) in five tissues(gonad,foot,mantle,siphon and adductor muscle) were analyzed.The glycogen content was high before gametogenesis,and decreased significantly during the gonad development in the gonad,mantle and foot of both females and males,suggesting that glycogen was an important energy source for gonad development.The protein and lipid contents increased in the ovary during the gonad development,demonstrating that they are the major organic components of oocytes.The lipid and protein contents decreased in the testis,implying that they can provide energy and material for spermatogenesis.The results also showed that protein stored in the mantle and foot could support the reproduction after the glycogen was depleted. Seasonal variation of biochemical components in clam (Saxidomus purpuratus Sowerby 1852) was investigated from March 2012 to February 2013 in relation to environmental condition of Sanggou Bay and the reproductive cycle of clam. According to the histological analysis, the reproductive cycle of S. purpuratus includes two distinctive phases: a total spent and inactive stage from November to January, and a gametogenesis stage, including ripeness and spawning, during the rest of the year. Gametes were generated at a low temperature (2.1 ° C) in February.Spawning took place once a year from June to October. The massive spawning occurred in August when the highest water temperature and chlorophyll a level could be observed. The key biochemical components (glycogen, protein and lipid) in five tissues (gonad, foot, mantle, siphon and adductor muscle) were analyzed. The glycogen content was high before gametogenesis, and decreased significantly during the gonad development in the gonad, mantle and foot of both femal es and males, suggesting that glycogen was an important energy source for gonad development.The protein and lipid contents increased in the ovary during the gonad development, demonstrating that they are the major organic components of oocytes.The lipid and protein contents decreased in the testis implying that they can provide energy and material for spermatogenesis. The results also showed that protein stored in the mantle and foot could support the reproduction after the glycogen was depleted.