论文部分内容阅读
目的:探讨恒定磁场联合阿霉素对诱导肝癌细胞凋亡的影响。方法:采用流式细胞仪碘化丙啶染色法和活细胞荧光染色法(Hoechst染色法),观察不同处理因素对细胞凋亡率的影响。结果:两种实验方法均显示05mg/L的阿霉素与对照组比较没有显著差异(P>005),而单独用恒定磁场或10mg/L的阿霉素与对照组比较有显著性差异(P<005)。应用恒定磁场后发现,05mg/L和10mg/L浓度的阿霉素诱导HepG-2细胞凋亡的结果与对照组相比,均有显著差异(P<005)。结论:恒定磁场具有增强阿霉素诱导肝癌细胞凋亡的作用
Objective: To investigate the effect of constant magnetic field combined with adriamycin on apoptosis of hepatoma cells. Methods: Flow cytometry propidium iodide staining and living cell fluorescence staining (Hoechst staining) were used to observe the effects of different treatment factors on the apoptosis rate. Results: Both experimental methods showed that there was no significant difference between 05mg/L adriamycin and the control group (P>005), but with constant magnetic field or 1.0 mg/L adriamycin alone and the control group. There was a significant difference (P<005). Using a constant magnetic field, it was found that the apoptosis of HepG-2 cells induced by doxorubicin at concentrations of 0.5 mg/L and 1.0 mg/L was significantly different from that of the control group (P < 0.05). Conclusion: The constant magnetic field can enhance the apoptosis of hepatoma cells induced by adriamycin