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目的 探讨PTEN基因在子宫内膜癌基因治疗方面的可行性。方法 通过腺病毒载体(Ad PTEN)将人野生型PTENcDNA导入Ishikawa细胞 ,观察外源性PTEN蛋白的表达情况 ,以及PTEN蛋白对Ishikawa细胞生长的影响 ,同时还观察了PTEN蛋白是否影响胰岛素样生长因子Ⅱ (IGF Ⅱ )对Ishikawa细胞的作用。结果 Ad PTEN感染Ishikawa细胞后 1d ,即可见PTEN蛋白表达 ;第 3天时 ,表达明显增强 ,并能持续表达 10d。感染后的Ishikawa细胞生长受到明显抑制 ,并且PTEN还能明显抑制IGF Ⅱ诱导的Ishikawa细胞生长。结论 腺病毒介导的PTEN基因导入能诱导Ishikawa细胞生长受到明显抑制 ,提示野生型PTEN基因可能是治疗子宫内膜癌的一种新途径。
Objective To investigate the feasibility of PTEN gene in endometrial cancer gene therapy. Methods Human wild type PTEN cDNA was transfected into Ishikawa cells by adenovirus vector (Ad PTEN) to observe the expression of exogenous PTEN protein and the effect of PTEN protein on the growth of Ishikawa cells. It was also observed whether PTEN protein affected insulin-like growth factor Ⅱ (IGF Ⅱ) on Ishikawa cells. Results The expression of PTEN protein was detected on the 1st day after Ad PTEN infection in Ishikawa cells. On the 3rd day, the expression of PTEN protein was significantly enhanced and persisted for 10 days. Infection of Ishikawa cells was significantly inhibited, and PTEN also significantly inhibited IGF Ⅱ induced Ishikawa cell growth. Conclusions Adenovirus-mediated PTEN gene transfection can significantly inhibit the growth of Ishikawa cells, suggesting that wild-type PTEN gene may be a new way to treat endometrial cancer.