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目的从整体水平探索幽门螺杆菌(Hp)感染后宿主的蛋白相互作用网络,挖掘其感染宿主细胞和导致宿主细胞病变的关键基因,为Hp感染的机制提供新的线索,可以为细菌-宿主联合靶向治疗提供基础。方法利用Hp感染的宿主细胞表达谱,基于激活子网算法构建了Hp感染激活子网,并对子网中的基因进行基因本体(GeneOntology,GO)分类。结果通过GO分类发现,子网中主要涉及宿主免疫反应的基因、细胞骨架相关基因和细胞增殖相关基因。结论幽门螺杆菌侵染宿主主要引发宿主的免疫反应、细菌进入宿主细胞的反应和宿主增殖与病变的反应。
OBJECTIVE: To explore the host protein-protein interaction network of Helicobacter pylori (Hp) infection and to find out the key genes in the infected host cells and the host cell pathological changes, and to provide new clues for the mechanism of Hp infection, which may be bacterial-host combination Targeted therapy to provide the foundation. Methods The host cell expression profile of Hp infection was used to construct the Hp infection activation sub-net based on the activation sub-net algorithm. The genes in the sub-net were classified by Gene Ontology (GO). Results According to the GO classification, it was found that genes in the subnet mainly involved in host immune response, cytoskeleton related genes and cell proliferation related genes. Conclusion Helicobacter pylori infection of the host mainly induced host immune response, bacterial entry into the host cell response and host proliferation and pathological response.