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目的 :建立快速测定人血浆中非诺贝特活性代谢物非诺贝酸 (fenofibricacid)的高效液相色谱方法。方法 :以乙腈 - 1mol·L-1盐酸 (95∶5 )直接沉淀血浆蛋白 ,色谱柱为配有Waters保护柱的YWG -ODS 10 μm 2 0 0mm× 4 0mm ,流动相为甲醇 -水 - 10 %磷酸 (76∶2 4∶1) ,检测波长 2 86nm ,外标法峰高定量。结果 :非诺贝酸的保留时间约为 4 4min ,定量线性范围 0 2 5～ 18 75 μg·mL-1,绝对回收率大于 85 % (n =5 ) ,方法回收率大于 90 % (n =5 ) ,日内日间RSD小于 10 % (n =5 )。结论 :本法简便快速、定量准确 ,适用于非诺贝特键康人体临床药代动力学研究
Objective: To establish a rapid HPLC method for the determination of fenofibric acid, the active metabolite of fenofibrate, in human plasma. Methods: The plasma protein was directly precipitated by acetonitrile - 1mol·L-1 hydrochloric acid (95: 5). The column was YWG-ODS 10 μm 200 mm × 40 mm with Waters guard column and the mobile phase was methanol-water- % Phosphoric acid (76: 2 4: 1), detection wavelength of 2 86nm, external standard peak high quantitative. Results: The retention time of fenofibrate was about 4 4 min, the linear range of quantitation was from 25 2 to 18 75 μg · mL -1, the absolute recovery was over 85% (n = 5), and the recovery was over 90% (n = 5), day RSD less than 10% (n = 5). Conclusion: This method is simple and rapid, accurate and accurate, suitable for fenofibrate key health pharmacokinetics