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目的研究中波紫外线(UVB)对人表皮朗格汉斯细胞(LC)的光损伤作用以及绿茶活性成分表没食子儿茶素没食子酸酯(EGCG)的光保护作用。方法采用密度梯度离心和免疫磁珠的方法分离纯化人表皮LC,将分离纯化的LC随机分为对照组、30 mJ/cm~2 UVB辐射组以及辐射后加用200μg/mL EGCG处理组,4h后以PI染色并经流式细胞仪检测细胞凋亡率。结果30 mJ/cm~2 UVB辐射组的凋亡率明显高于对照组,EGCG干预组的凋亡率低于单纯UVB辐射组,但仍高于非照射对照组；且辐射后S期细胞数明显增加。几乎没有G)2/M期的细胞,EGCG处理辐射细胞后,S期细胞数减少。结论UVB可诱导人表皮LC凋亡,而EGCG具有一定的保护作用。
Objective To study the photodamage effects of ultraviolet (UVB) on human epidermal Langerhans cells (LC) and the photoprotective effects of the green tea active ingredient epigallocatechin gallate (EGCG). Methods Human epidermal LCs were isolated and purified by density gradient centrifugation and immunomagnetic beads. The isolated and purified LCs were randomly divided into control group, 30 mJ/cm~2 UVB radiation group and 200 μg/mL EGCG treatment group after radiation, 4 h. After staining with PI, the apoptosis rate was detected by flow cytometry. Results The apoptotic rate of 30 mJ/cm~2 UVB radiation group was significantly higher than that of the control group. The apoptosis rate of the EGCG intervention group was lower than that of the single UVB radiation group, but still higher than that of the non-irradiation control group; and the number of S phase cells after irradiation. obviously increase. Almost no cells in G2/G2 phase treated with EGCG reduced the number of cells in S phase. Conclusion UVB can induce apoptosis of human epidermal LC, while EGCG has a protective effect.