论文部分内容阅读
[目的]研究制备双光子蛋白质荧光探针的中间产物2,5-二溴对二甲苯的合成条件.[方法]以对二甲苯为原料,在控制物料配比、溶液浓度、反应时间的条件下通过变换紫外线照射与反应温度等因素探索合成2,5-二溴对二甲苯的最适合条件.[结果]紫外线照射可降低反应的重复性,2,5-二溴对二甲苯产率为37.1%±22.7%;在无紫外线照射且避光环境下,0℃,10~15℃,19~24℃及40℃时,2,5-二溴对二甲苯的产率分别为43.60%±3.12%,53.13%±3.79%,50.83%±9.83%,43.23%±6.84%,在10~15℃时2,5-二溴对二甲苯的产率最高(P<0.05).[结论]在避光条件下,循环水控制温度为10~15℃是合成2,5-二溴对二甲苯的最适合条件.
[Objective] The research aimed to study the synthesis conditions of 2,5-dibromo-p-xylene, an intermediate of the two-photon protein fluorescent probe. [Method] With p-xylene as raw material, conditions of material ratio, solution concentration and reaction time The optimal conditions for the synthesis of 2,5-dibromo-p-xylene were explored by changing the UV irradiation and the reaction temperature. [Result] The UV irradiation could reduce the repeatability of the reaction. The yield of 2,5-dibromo-p- 37.1% ± 22.7%. The yields of 2,5-dibromo-p-xylene at 0 ℃, 10 ~ 15 ℃, 19 ~ 24 ℃ and 40 ℃ were 43.60% ± 3.12%, 53.13% ± 3.79%, 50.83% ± 9.83% and 43.23% ± 6.84%, respectively. The yield of 2,5-dibromo-p-xylene was the highest at 10 ~ 15 ℃ Under dark conditions, the optimum conditions for the synthesis of 2,5-dibromo-p-xylene are that the circulating water control temperature is 10-15 ° C.