论文部分内容阅读
目的:观察乙乙乳膏对醋酸所致小鼠疼痛模型的影响。方法:取小鼠48只,随机分为4组各12只,即乙乙乳膏高、低剂量组、空白组和扶他林对照组。将药物涂于小鼠尾部,面积为1cm2,每天给药1次,连续给药7天,空白组只贴乳膏。于第7天所有小鼠腹腔注射0.6%醋酸溶液0.2mL/10g致痛,记录各小鼠出现第1次扭体反应的时间及15min内扭体次数,比较各组扭体次数。结果:乙乙乳膏高、低剂量组平均扭体次数(16.4±8.5)和(19.2±11.4)次,均显著少于空白组(23.4±12.2)。乙乙乳膏各剂量组与扶他林剂量组(15.8±8.6)次比较,扭体次数无显著差异。乙乙乳膏高、低剂量组扭体反应的抑制率分别为29.9%和19.9%。扶他林组扭体反应的抑制率为32.5%。乙乙乳膏组平均扭体次数均较空白组少(均P<0.01)。结论:乙乙乳膏对醋酸所致小鼠疼痛模型有明显的镇痛作用,且镇痛作用与剂量呈量效关系。
OBJECTIVE: To observe the effect of E-B cream on acetic acid-induced pain model in mice. METHODS: Forty-eight mice were randomly divided into 4 groups, 12 in each group, namely high- and low-dose group of E-B cream, blank group and control group. The drug was applied to the tail of the mice, and the area was 1 cm 2 , which was administered once a day for 7 days. The blank group was given only cream. On the 7th day, all mice were intraperitoneally injected with 0.2% acetic acid solution (0.2mL/10g) to induce pain. The time of the first writhing reaction and the number of writhings in 15 minutes were recorded in each mouse. The number of writhings in each group was compared. RESULTS: The average writhing frequency of high and low doses of E-B cream was (16.4±8.5) and (19.2±11.4) times, which were significantly lower than that of the blank group (23.4±12.2). There was no significant difference in the number of writhing between the doses of E-B cream and the doses of Futaline (15.8±8.6). The inhibitory rates of writhing responses in the high and low doses of E-B cream were 29.9% and 19.9%, respectively. The inhibition rate of the writhing response in the futalin group was 32.5%. The average number of writhing in the group B was lower than that in the blank group (all P<0.01). Conclusion: E-B cream has obvious analgesic effect on acetic acid-induced pain model in mice, and the analgesic effect is dose-effect relationship with dose.