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为探讨联合追踪急性淋巴细胞白血病（ALL）患儿骨髓（BM）、外周血（PB）、脑脊液（CSF）等多种微小残留病（MRD）的临床意义。采用巢式PCR法检测28例ALL患儿，对其中14例联合追踪完全缓解（CR）后BM、PB、CSF多种标本MRD的消长。结果BM、PB、CSF标本在CR后一段时间有MRD存在，但分布不均，随访14例中，8例CR后BM、PB、CSFMRD渐少者呈持续缓解，而4例BM的MRD持续阳性或由阴转归者骨髓复发，CSF的MRD1例持续阳性，另1例由阴转阳性，发生中枢神经系统自血病（CNSL）。提示多标本联合追踪急淋缓解期MRD对指导治疗、预测复发有重要意义。
To explore the clinical significance of multiple trace residual diseases (MRD) such as bone marrow (BM), peripheral blood (PB), and cerebrospinal fluid (CSF) in children with acute lymphoblastic leukemia (ALL). By nested PCR detection of 28 cases of ALL children, of which 14 cases of complete tracking of complete remission (CR) after BM, PB, CSF multi-sample MRD growth. Results There were MRDs in BM, PB, and CSF samples after CR, but the distribution was uneven. In 14 cases of follow-up, BM, PB, and CSFMRD were gradually reduced after CR in 8 cases, and MRD of 4 cases of BM continued to be positive. Or the bone marrow relapsed from the infarct, the MRD1 case of CSF was persistently positive, and the other case was negative to positive, resulting in central nervous system autoimmune disease (CNSL). This suggests that multi-specimen joint tracking of acute lymphocytic MRD is important for guiding treatment and predicting recurrence.