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作者以小鼠的抗班氏丝虫L3期感染性幼虫的抗血清用免疫筛选法筛选cDNA库,分离出马来丝虫L3副肌球蛋白的cDNA全长的编码序列,谷胱甘肽-S-转移酶溶合蛋白是副肌球蛋白的重组切断模式。研究对象为法国玻利尼亚地区一个班氏丝虫流行区的123名患者,阴性对照为九名欧洲人。所有患者均接受2次化学治疗,每年一次伊维菌素400μg/kg加海群生3mg/kg,在第一次治疗前(0月),一年后第二次治疗前(12月),第二次治疗一年后(24月)采血,三次均镜检微丝蚴(Mf)和循环丝虫抗原(CFA)。用ELISA跟踪检测流行区患者及阴性对照的抗重组切断副肌球蛋白(truncated paramyosin)抗体IgG总量及其亚型的含量。
In this study, we screened the cDNA library by antiserum against L3 larvae infected with Bancroftian filares by immunofluorescence. The full-length coding sequence of L3 paramyxovirus was isolated and the coding sequence of glutathione-S - Transferase Fusions are recombinant shuttling patterns of paramyosin. The study population was 123 patients in a Bancrofoo-endemic area in the French province of Bolivia, with nine Europeans as the negative control. All patients received 2 chemotherapy, once a year ivermectin 400μg / kg plus sea lump 3mg / kg, before the first treatment (0 months), one year after the second treatment (December), the first One year after the second treatment (24 months), blood was taken three times and microfilariae (Mf) and circulating filarial antigen (CFA) were examined microscopically. The total amount of anti-recombinant truncated paramyosin IgG and its subtypes in patients in the endemic area and the negative control were detected by ELISA.