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本文叙述了用GC-MS联用技术研究脱氢氯甲睾酮人体内代谢的方法。尿样中的甾体化合物经大孔树脂吸附提取后,酶解。浓缩并衍生化,进行GC-MS分析。在服药后8～30h的尿样中,检出了脱氢氯甲睾酮原型,并发现了七个代谢产物。分析色谱和质谱数据,得到了这些代谢物的结构及其浓度变化趋势,推测了脱氢氯甲睾酮可能的体内代谢模式,确定了筛检尿中脱氢氯甲睾酮的特定代谢物和特征检测离子,比较了不同的样品处理方法对分析结果的影响。
This article describes the GC-MS combined with dehydrochlorothiazide testosterone metabolism in humans. Urine samples of steroids by macroporous resin adsorption extraction, enzymatic hydrolysis. Concentrated and derivatized for GC-MS analysis. In urine samples taken 8 to 30 hours after dosing, the prototype of dehydrochloromethyltestosterone was detected and seven metabolites were found. Chromatographic and mass spectrometric data were analyzed and the structure and concentration trends of these metabolites were obtained. The possible in vivo metabolism of demethoxydicarb was estimated, and the specific metabolites and characteristics of dechloromethyltestosterone in urine were screened Ions, comparing the impact of different sample processing methods on the analysis results.