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已报道的磺胺类药物分离方法有离子交换色谱、离子对色谱和吸附色谱。测定方法有重氮化滴定法、薄层扫描,但专属性不好,测定时间较长。本文利用环糊精和磺胺类药物在溶液中能相互作用,以环糊精(β-CyD)为键合固定相,用HPLC 法测定制剂中磺胺类药物的含量。条件与试样:Du pont 8800泵、Du pont紫外-可见检测器,SP 4100记录仪。检测波长:257nm。流动相:磷酸缓冲液(pH7.0)-甲醇(85:15),流速:0.5ml/min,纸速:0.25cm/min。三磺嘧啶片含磺胺嘧啶、磺胺
Reported methods for the separation of sulfonamides are ion exchange chromatography, ion pair chromatography and adsorption chromatography. Determination of diazotization titration, TLC, but the specificity is not good, the determination of longer. In this paper, cyclodextrins and sulfonamides were used to interact with each other in solution. Cyclodextrin (β-CyD) was used as the stationary phase. The contents of sulfonamides in the preparations were determined by HPLC. Conditions and samples: Du pont 8800 pump, Du pont UV-Vis detector, SP 4100 recorder. Detection wavelength: 257nm. Mobile phase: Phosphate buffer (pH 7.0) -methanol (85:15), flow rate: 0.5 ml / min, paper speed: 0.25 cm / min. Sulfathine tablets containing sulfadiazine, sulfonamides