本文用19日～21日胎龄的SD种胎鼠的后肢肌肉与同种大鼠骨基质明胶一起做体外诱导培养,培非液为含3 700 mg/L NaHCO_3的DMEM。培养10日时,8个培养物中有7个出现诱导性软骨细胞。培养15、20、25日时,30个培养物(每个时间点10个培养物)中都出现了诱导性软骨细胞。结果表明,本实验所采用的组织培养方法和含3 700 mg/L NaHCO_3的DMEM培养液完全适用于体外诱导软骨细胞形成。 In this paper, the hindlimb muscle of SD fetus with gestational age from 19 to 21 days was cultured in vitro with the same rat bone matrix gelatin. The culture medium was DMEM containing 3700 mg / L NaHCO 3. Induced chondrocytes appeared in 7 out of 8 cultures on the 10th day of culture. Induced chondrocytes appeared in 30 cultures (10 cultures at each time point) at 15, 20, and 25 days of culture. The results showed that the tissue culture method and DMEM culture medium containing 3700 mg / L NaHCO 3 were completely suitable for inducing chondrocyte formation in vitro.