Surface soil samples collected over a high spatial resolution in eastern China were analyzed for carbon isotope composition(δ 13C) of total organic carbon(TOC) and higher plant-derived long-chain n-alkanes,with the latter reported as weighted mean values.The two sets of δ 13C values are significantly correlated and show similar trends in spatial variation.The spatial distribution of δ 13C shows less negative values in the mid-latitudes between 31°N and 40°N and more negative ones at higher and lower latitudes.This is consistent with previously reported carbon isotope data from surface soil phytoliths in the same region and suggests that the mid-latitude area provides relatively favorable growing conditions for C4 plants.Furthermore,δ 13C values of both TOC and long-chain n-alkanes from 12 surface soil samples collected from a small grassland in north China displayed similar carbon isotope values and the difference between paired δ 13C of a soil samples remains relatively constant.Our data demonstrate that in eastern China,soil δ 13C composition of both TOC and long-chain n-alkanes is effective indicators of C3/C4 ratios of the prevailing vegetation.This work suggests that -22‰ and -32‰ are good estimated end members for the weighted mean δ 13C values of long-chain n-alkanes(C27,C29 and C31 n-alkanes) from soils under dominant C4 or C3 vegetation,allowing us to reconstruct paleovegetation trends. Surface soil samples collected over a high spatial resolution in eastern China were analyzed for carbon isotope composition (δ 13C) of total organic carbon (TOC) and higher plant-derived long-chain n-alkanes, with the latter reported as weighted mean values. The two sets of δ 13C values ​​are both obvious correlated and show similar trends in spatial variation. Δ 13C shows less negative values ​​in the mid-latitudes between 31 ° N and 40 ° N and more negative ones at higher and lower latitudes .This is consistent with previously reported carbon isotope data from surface soil phytoliths in the same region and suggests that the mid-latitude area provides less favorable growing conditions for C4 plants. Further, δ 13C values ​​of both TOC and long-chain n-alkanes from 12 surface soil samples collected from a small grassland in north China displayed similar carbon isotope values ​​and the difference between paired δ 13C of a soil samples remains relatively constan t.Our data demonstrates that in eastern China, soil δ 13C composition of both TOC and long-chain n-alkanes is effective indicators of C3 / C4 ratios of the prevailing vegetation. This work suggests that -22 ‰ and -32 ‰ are good estimated end members for the weighted mean δ 13C values ​​of long-chain n-alkanes (C27, C29 and C31 n-alkanes) from soils under dominant C4 or C3 vegetation, allowing us to reconstruct paleovegetation trends.